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synaptic vesicles sv2  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank synaptic vesicles sv2
    Synaptic Vesicles Sv2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 98/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+sv2/anti-Synaptic+vesicle+glycoprotein+2A/pm42140979-205-34-38
    Average 98 stars, based on 128 article reviews
    synaptic vesicles sv2 - by Bioz Stars, 2026-10
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    Article Title: Impact of maternal compensation on developmental phenotypes in a zebrafish model of severe congenital muscular dystrophy
    Article Snippet: All cryosections were imaged on a Zeiss LSM800 confocal microscope with Zeiss Zen imaging software.

    Article Title: Impact of maternal compensation on developmental phenotypes in a zebrafish model of severe congenital muscular dystrophy.
    Article Snippet: The fish were then transferred to 15% sucrose with 0.2% sodium azide in 1X PBS for 1–2 days, followed by 30% sucrose with 0.2% sodium azide in 1X PBS for 1–2 days, and then frozen in isopentane on dry ice in Tissue Freezing Medium (Ted Pella Inc) and stored at -80°C.

    Article Title: Semaphorin 7A Regulates Axon Outgrowth in Subcutaneous White Adipose Tissue.
    Article Snippet: Secondary antibodies included: Goat anti‐167 Jo ur al Pr e-p r o f mouse IgG1 Alexa Fluor 647 (1:500, Invitrogen, Cat#A‐21240).


    Blocking Assay:

    Article Title: Early motor deficits, sleep dysfunction and reduction in dopaminergic neurons in a PARK7 -/- zebrafish larval model of Parkinson’s disease
    Article Snippet: .. Clearing was performed according to Pende et al. . Larvae were then transferred to blocking solution (5% normal goat, 1% BSA and 0.1% Triton X) for 3 h at RT and incubated for 3 days with either anti-tyrosine hydroxylase (TH) (1:250, Merck Millipore MAB318) or anti-SV2 (1:1000, DSHB SV2-C) in antibody dilution buffer (5% goat serum, 1% BSA and 0.1% Triton-X in PBS) at 4 °C with gentle agitation.. ..

    Article Title: Early motor deficits, sleep dysfunction and reduction in dopaminergic neurons in a PARK7 -/- zebrafish larval model of Parkinson's disease.
    Article Snippet: .. Larvae were then transferred to blocking solution (5% normal goat, 1%BSA and 0.1% Triton X) for 3 hrs at RT and incubated for 3 days with either anti-tyrosine hydroxylase (TH) (1:250, Merck Millipore MAB318) or anti-SV2 (1:1000, DSHB SV2-C) in antibody dilution buffer (5% goat serum, 1% BSA and 0.1% Triton-X in PBS) at 4 oC with gentle agitation.. ..

    Incubation:

    Article Title: Early motor deficits, sleep dysfunction and reduction in dopaminergic neurons in a PARK7 -/- zebrafish larval model of Parkinson’s disease
    Article Snippet: .. Clearing was performed according to Pende et al. . Larvae were then transferred to blocking solution (5% normal goat, 1% BSA and 0.1% Triton X) for 3 h at RT and incubated for 3 days with either anti-tyrosine hydroxylase (TH) (1:250, Merck Millipore MAB318) or anti-SV2 (1:1000, DSHB SV2-C) in antibody dilution buffer (5% goat serum, 1% BSA and 0.1% Triton-X in PBS) at 4 °C with gentle agitation.. ..

    Article Title: Early motor deficits, sleep dysfunction and reduction in dopaminergic neurons in a PARK7 -/- zebrafish larval model of Parkinson's disease.
    Article Snippet: .. Larvae were then transferred to blocking solution (5% normal goat, 1%BSA and 0.1% Triton X) for 3 hrs at RT and incubated for 3 days with either anti-tyrosine hydroxylase (TH) (1:250, Merck Millipore MAB318) or anti-SV2 (1:1000, DSHB SV2-C) in antibody dilution buffer (5% goat serum, 1% BSA and 0.1% Triton-X in PBS) at 4 oC with gentle agitation.. ..

    Gentle:

    Article Title: Early motor deficits, sleep dysfunction and reduction in dopaminergic neurons in a PARK7 -/- zebrafish larval model of Parkinson’s disease
    Article Snippet: .. Clearing was performed according to Pende et al. . Larvae were then transferred to blocking solution (5% normal goat, 1% BSA and 0.1% Triton X) for 3 h at RT and incubated for 3 days with either anti-tyrosine hydroxylase (TH) (1:250, Merck Millipore MAB318) or anti-SV2 (1:1000, DSHB SV2-C) in antibody dilution buffer (5% goat serum, 1% BSA and 0.1% Triton-X in PBS) at 4 °C with gentle agitation.. ..

    Article Title: Early motor deficits, sleep dysfunction and reduction in dopaminergic neurons in a PARK7 -/- zebrafish larval model of Parkinson's disease.
    Article Snippet: .. Larvae were then transferred to blocking solution (5% normal goat, 1%BSA and 0.1% Triton X) for 3 hrs at RT and incubated for 3 days with either anti-tyrosine hydroxylase (TH) (1:250, Merck Millipore MAB318) or anti-SV2 (1:1000, DSHB SV2-C) in antibody dilution buffer (5% goat serum, 1% BSA and 0.1% Triton-X in PBS) at 4 oC with gentle agitation.. ..

    Staining:

    Article Title: Myoblast Therapy Ameliorates Skeletal Muscle Atrophy Resulting From Chronic Denervation.
    Article Snippet: Introduction/Aims: Skeletal muscle undergoes progressive denervationinduced muscle atrophy (DIMA) after peripheral nerve injury that severely impairs the potential for motor functional recovery with reinnervation.. There are currently no therapeutic strategies to reverse the deleterious effects of chronic DIMA, leaving affected patients with lifelong disability.. Herein, we used a translational rodent forelimb nerve injury model to investigate whether targeted injection of syngeneic myoblasts to chronically atrophic muscle can reverse the histologic and functional consequences of DIMA.



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    ( A ) Schematic of the construct used to make the UAS:syp-dL5-mNeonGreen transgenic line. ( B ) Horizontal confocal section through the spinal cord of 3-dpf y417-Gal4, UAS:syp-dL5-mNeonGreen embryo (green), stained with <t>anti-sv2</t> (magenta). Scale bar, 20 μm. Arrows show synaptic puncta from y417 neurons. “A” and “D” denote anterior-dorsal orientation. The boxed region is shown at a higher magnification in the right panel. Scale bar, 5 μm. ( C and D ) Maximum projection from the confocal z -stack of GFP and differential interference contrast channels in 4 dpf y252-Gal4, UAS:syp-dL5-mNeonGreen larvae: untreated control larva (C) and an MG-2I–treated larva exposed for 20 min to wide-field illumination at 3 dpf (D). Scale bar, 100 μm. A square ROI was cropped from each panel in (C) and (D) and is shown at a higher magnification in (Ca) and (Da). ( E ) SLC [ n = 25 and 25 for ctl (dL5 − /Mg2I + ) and abl (dL5 + /Mg2I + )] and LLC ( n = 25 and 20) escape responsiveness and PPI ( n = 14 and 16) in control and wide-field light–exposed y252-Gal4, UAS:syp-dL5-mNeonGreen larvae. MWU, significant P value indicated and * P < 0.001. ( F ) Maximum projection of 80-μm confocal stacks from 4-dpf tph2:Gal4, UAS:syp-dL5, UAS:Kaede Red untreated larvae and in larvae exposed to NIR illumination in the indicated region of the left hindbrain at 3 dpf. Scale bars, 100 μm. Ra, raphe neurons. ( G ) Number of neurons in dorsal raphe 24 hours after photoablation of neuropil in the region indicated in (F) in untreated [ctl (dL5 − /Mg2I + ), n = 9] and photoablated larvae [abl (dL5 + /Mg2I + ), n = 8]. Welch t test, P = 0.50.
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    DAB1 overexpression induces axonal defects during development (A) Schematic representation of cerebellar and cerebellar-like structures in zebrafish. Granule cell (GC) axons, named parallel fibers (PFs), innervate Purkinje cells (PCs) in the molecular cell layer. Axons from GCs in eminentia granularis (EG) innervate a type of Purkinje-like neuron called crest cells (CrCs). (B) Representative confocal images of zebrafish larvae, from a GFP + cerebellar granule cell line, microinjected with control or human DAB1 mRNA at 5 dpf; axonal tracts of GCs from EG are indicated with orange (absent) or gray (present) arrows. (C) Percentage of present or absent GC axonal tracts that innervate PC-like neurons (graphic representation of data by mean ± SD of at least 20 embryos/condition/replicate; three independent experiments; ꭕ 2 test for the percentage of GC axonal tracts, present or absent; ∗∗∗ p < 0.001). (D) Schematics of muscle innervation by PMN axon tracts in zebrafish larvae at 24 hpf. (E) Representative confocal images (40× objective) of PMN axon tracts from the 6-somites-spanning region anterior to the cloaca from control or DAB1 mRNA-injected embryos; whole-mounted immunofluorescence with <t>SV2</t> antibody, representative z-projection images (maximum intensity). (F) Length of zebrafish PMN axon tracts in control and DAB1 mRNA-microinjected embryos ( n = 23 embryos for control and n = 24 embryos in DAB1 mRNA condition; four independent experiments; independent t test, ∗ p < 0.05; data are represented by the mean ± SD). (G) Proposed mechanistic model showing disruption of DRL function by the (ATTTC) n , which hyperactivates the neurodevelopmental enhancer, leading to DAB1 upregulation and DAB1 protein accumulation in axons, likely contributing to SCA37. See also .
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    DAB1 overexpression induces axonal defects during development (A) Schematic representation of cerebellar and cerebellar-like structures in zebrafish. Granule cell (GC) axons, named parallel fibers (PFs), innervate Purkinje cells (PCs) in the molecular cell layer. Axons from GCs in eminentia granularis (EG) innervate a type of Purkinje-like neuron called crest cells (CrCs). (B) Representative confocal images of zebrafish larvae, from a GFP + cerebellar granule cell line, microinjected with control or human DAB1 mRNA at 5 dpf; axonal tracts of GCs from EG are indicated with orange (absent) or gray (present) arrows. (C) Percentage of present or absent GC axonal tracts that innervate PC-like neurons (graphic representation of data by mean ± SD of at least 20 embryos/condition/replicate; three independent experiments; ꭕ 2 test for the percentage of GC axonal tracts, present or absent; ∗∗∗ p < 0.001). (D) Schematics of muscle innervation by PMN axon tracts in zebrafish larvae at 24 hpf. (E) Representative confocal images (40× objective) of PMN axon tracts from the 6-somites-spanning region anterior to the cloaca from control or DAB1 mRNA-injected embryos; whole-mounted immunofluorescence with <t>SV2</t> antibody, representative z-projection images (maximum intensity). (F) Length of zebrafish PMN axon tracts in control and DAB1 mRNA-microinjected embryos ( n = 23 embryos for control and n = 24 embryos in DAB1 mRNA condition; four independent experiments; independent t test, ∗ p < 0.05; data are represented by the mean ± SD). (G) Proposed mechanistic model showing disruption of DRL function by the (ATTTC) n , which hyperactivates the neurodevelopmental enhancer, leading to DAB1 upregulation and DAB1 protein accumulation in axons, likely contributing to SCA37. See also .
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    DAB1 overexpression induces axonal defects during development (A) Schematic representation of cerebellar and cerebellar-like structures in zebrafish. Granule cell (GC) axons, named parallel fibers (PFs), innervate Purkinje cells (PCs) in the molecular cell layer. Axons from GCs in eminentia granularis (EG) innervate a type of Purkinje-like neuron called crest cells (CrCs). (B) Representative confocal images of zebrafish larvae, from a GFP + cerebellar granule cell line, microinjected with control or human DAB1 mRNA at 5 dpf; axonal tracts of GCs from EG are indicated with orange (absent) or gray (present) arrows. (C) Percentage of present or absent GC axonal tracts that innervate PC-like neurons (graphic representation of data by mean ± SD of at least 20 embryos/condition/replicate; three independent experiments; ꭕ 2 test for the percentage of GC axonal tracts, present or absent; ∗∗∗ p < 0.001). (D) Schematics of muscle innervation by PMN axon tracts in zebrafish larvae at 24 hpf. (E) Representative confocal images (40× objective) of PMN axon tracts from the 6-somites-spanning region anterior to the cloaca from control or DAB1 mRNA-injected embryos; whole-mounted immunofluorescence with <t>SV2</t> antibody, representative z-projection images (maximum intensity). (F) Length of zebrafish PMN axon tracts in control and DAB1 mRNA-microinjected embryos ( n = 23 embryos for control and n = 24 embryos in DAB1 mRNA condition; four independent experiments; independent t test, ∗ p < 0.05; data are represented by the mean ± SD). (G) Proposed mechanistic model showing disruption of DRL function by the (ATTTC) n , which hyperactivates the neurodevelopmental enhancer, leading to DAB1 upregulation and DAB1 protein accumulation in axons, likely contributing to SCA37. See also .
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    Motor function (latency to fall from an inverted screen) measured in 3-month-old WT, TDP43 Q331K/+ , LDHB MNKO and TDP43 Q331K/+ ; LDHB MNKO mice (A), electrophysiological recording performed on 3-month-old animals to determine the difference in CMAP at the ankle and sciatic notch ( B & C ) and Sensory Nerve Action Potential (SNAP) at the tail (n = 10-12) (D). NMJs on lumbrical muscles stained with <t>anti-NF,</t> <t>anti-SV2</t> and BTX to compare innervation of 3-month-old mice of different genotypes (n = 3; statistical comparison is between fully innervated endplates). Statistical significance was determined by pairwise Fisher’s exact tests on raw counts, with a Bonferroni correction applied for multiple comparisons. (E-I). Spinal cords stained to detect TDP43 and nuclei (DAPI) indicate no mislocalization in 6-month-old LDHB MNKO; TDP43 Q331K/+ or LDHB MNKO mice (J & K) . Representative images of 40x and 63x toluidine blue-stained sections of tibial nerves from 3-month-old animals (L-O) . Average g-ratio indicates no difference in degeneration between genotypes (n = 3). Unless otherwise mentioned, all statistical significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (P) . ***p < 0.001, **p < 0.01.
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    Motor function (latency to fall from an inverted screen) measured in 3-month-old WT, TDP43 Q331K/+ , LDHB MNKO and TDP43 Q331K/+ ; LDHB MNKO mice (A), electrophysiological recording performed on 3-month-old animals to determine the difference in CMAP at the ankle and sciatic notch ( B & C ) and Sensory Nerve Action Potential (SNAP) at the tail (n = 10-12) (D). NMJs on lumbrical muscles stained with <t>anti-NF,</t> <t>anti-SV2</t> and BTX to compare innervation of 3-month-old mice of different genotypes (n = 3; statistical comparison is between fully innervated endplates). Statistical significance was determined by pairwise Fisher’s exact tests on raw counts, with a Bonferroni correction applied for multiple comparisons. (E-I). Spinal cords stained to detect TDP43 and nuclei (DAPI) indicate no mislocalization in 6-month-old LDHB MNKO; TDP43 Q331K/+ or LDHB MNKO mice (J & K) . Representative images of 40x and 63x toluidine blue-stained sections of tibial nerves from 3-month-old animals (L-O) . Average g-ratio indicates no difference in degeneration between genotypes (n = 3). Unless otherwise mentioned, all statistical significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (P) . ***p < 0.001, **p < 0.01.
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    Motor function (latency to fall from an inverted screen) measured in 3-month-old WT, TDP43 Q331K/+ , LDHB MNKO and TDP43 Q331K/+ ; LDHB MNKO mice (A), electrophysiological recording performed on 3-month-old animals to determine the difference in CMAP at the ankle and sciatic notch ( B & C ) and Sensory Nerve Action Potential (SNAP) at the tail (n = 10-12) (D). NMJs on lumbrical muscles stained with <t>anti-NF,</t> <t>anti-SV2</t> and BTX to compare innervation of 3-month-old mice of different genotypes (n = 3; statistical comparison is between fully innervated endplates). Statistical significance was determined by pairwise Fisher’s exact tests on raw counts, with a Bonferroni correction applied for multiple comparisons. (E-I). Spinal cords stained to detect TDP43 and nuclei (DAPI) indicate no mislocalization in 6-month-old LDHB MNKO; TDP43 Q331K/+ or LDHB MNKO mice (J & K) . Representative images of 40x and 63x toluidine blue-stained sections of tibial nerves from 3-month-old animals (L-O) . Average g-ratio indicates no difference in degeneration between genotypes (n = 3). Unless otherwise mentioned, all statistical significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (P) . ***p < 0.001, **p < 0.01.
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    Image Search Results


    ( A ) Schematic of the construct used to make the UAS:syp-dL5-mNeonGreen transgenic line. ( B ) Horizontal confocal section through the spinal cord of 3-dpf y417-Gal4, UAS:syp-dL5-mNeonGreen embryo (green), stained with anti-sv2 (magenta). Scale bar, 20 μm. Arrows show synaptic puncta from y417 neurons. “A” and “D” denote anterior-dorsal orientation. The boxed region is shown at a higher magnification in the right panel. Scale bar, 5 μm. ( C and D ) Maximum projection from the confocal z -stack of GFP and differential interference contrast channels in 4 dpf y252-Gal4, UAS:syp-dL5-mNeonGreen larvae: untreated control larva (C) and an MG-2I–treated larva exposed for 20 min to wide-field illumination at 3 dpf (D). Scale bar, 100 μm. A square ROI was cropped from each panel in (C) and (D) and is shown at a higher magnification in (Ca) and (Da). ( E ) SLC [ n = 25 and 25 for ctl (dL5 − /Mg2I + ) and abl (dL5 + /Mg2I + )] and LLC ( n = 25 and 20) escape responsiveness and PPI ( n = 14 and 16) in control and wide-field light–exposed y252-Gal4, UAS:syp-dL5-mNeonGreen larvae. MWU, significant P value indicated and * P < 0.001. ( F ) Maximum projection of 80-μm confocal stacks from 4-dpf tph2:Gal4, UAS:syp-dL5, UAS:Kaede Red untreated larvae and in larvae exposed to NIR illumination in the indicated region of the left hindbrain at 3 dpf. Scale bars, 100 μm. Ra, raphe neurons. ( G ) Number of neurons in dorsal raphe 24 hours after photoablation of neuropil in the region indicated in (F) in untreated [ctl (dL5 − /Mg2I + ), n = 9] and photoablated larvae [abl (dL5 + /Mg2I + ), n = 8]. Welch t test, P = 0.50.

    Journal: Science Advances

    Article Title: Functional interrogation of neuronal connections by chemoptogenetic presynaptic ablation

    doi: 10.1126/sciadv.aeb6755

    Figure Lengend Snippet: ( A ) Schematic of the construct used to make the UAS:syp-dL5-mNeonGreen transgenic line. ( B ) Horizontal confocal section through the spinal cord of 3-dpf y417-Gal4, UAS:syp-dL5-mNeonGreen embryo (green), stained with anti-sv2 (magenta). Scale bar, 20 μm. Arrows show synaptic puncta from y417 neurons. “A” and “D” denote anterior-dorsal orientation. The boxed region is shown at a higher magnification in the right panel. Scale bar, 5 μm. ( C and D ) Maximum projection from the confocal z -stack of GFP and differential interference contrast channels in 4 dpf y252-Gal4, UAS:syp-dL5-mNeonGreen larvae: untreated control larva (C) and an MG-2I–treated larva exposed for 20 min to wide-field illumination at 3 dpf (D). Scale bar, 100 μm. A square ROI was cropped from each panel in (C) and (D) and is shown at a higher magnification in (Ca) and (Da). ( E ) SLC [ n = 25 and 25 for ctl (dL5 − /Mg2I + ) and abl (dL5 + /Mg2I + )] and LLC ( n = 25 and 20) escape responsiveness and PPI ( n = 14 and 16) in control and wide-field light–exposed y252-Gal4, UAS:syp-dL5-mNeonGreen larvae. MWU, significant P value indicated and * P < 0.001. ( F ) Maximum projection of 80-μm confocal stacks from 4-dpf tph2:Gal4, UAS:syp-dL5, UAS:Kaede Red untreated larvae and in larvae exposed to NIR illumination in the indicated region of the left hindbrain at 3 dpf. Scale bars, 100 μm. Ra, raphe neurons. ( G ) Number of neurons in dorsal raphe 24 hours after photoablation of neuropil in the region indicated in (F) in untreated [ctl (dL5 − /Mg2I + ), n = 9] and photoablated larvae [abl (dL5 + /Mg2I + ), n = 8]. Welch t test, P = 0.50.

    Article Snippet: Primary antibody incubation with a mouse anti–SV2 antibody (1:200, Developmental Studies Hybridoma Bank, Iowa City, IA) in PBS-T (PBS and 1% Triton) for 2 days at 4°C.

    Techniques: Construct, Transgenic Assay, Staining, Control

    DAB1 overexpression induces axonal defects during development (A) Schematic representation of cerebellar and cerebellar-like structures in zebrafish. Granule cell (GC) axons, named parallel fibers (PFs), innervate Purkinje cells (PCs) in the molecular cell layer. Axons from GCs in eminentia granularis (EG) innervate a type of Purkinje-like neuron called crest cells (CrCs). (B) Representative confocal images of zebrafish larvae, from a GFP + cerebellar granule cell line, microinjected with control or human DAB1 mRNA at 5 dpf; axonal tracts of GCs from EG are indicated with orange (absent) or gray (present) arrows. (C) Percentage of present or absent GC axonal tracts that innervate PC-like neurons (graphic representation of data by mean ± SD of at least 20 embryos/condition/replicate; three independent experiments; ꭕ 2 test for the percentage of GC axonal tracts, present or absent; ∗∗∗ p < 0.001). (D) Schematics of muscle innervation by PMN axon tracts in zebrafish larvae at 24 hpf. (E) Representative confocal images (40× objective) of PMN axon tracts from the 6-somites-spanning region anterior to the cloaca from control or DAB1 mRNA-injected embryos; whole-mounted immunofluorescence with SV2 antibody, representative z-projection images (maximum intensity). (F) Length of zebrafish PMN axon tracts in control and DAB1 mRNA-microinjected embryos ( n = 23 embryos for control and n = 24 embryos in DAB1 mRNA condition; four independent experiments; independent t test, ∗ p < 0.05; data are represented by the mean ± SD). (G) Proposed mechanistic model showing disruption of DRL function by the (ATTTC) n , which hyperactivates the neurodevelopmental enhancer, leading to DAB1 upregulation and DAB1 protein accumulation in axons, likely contributing to SCA37. See also .

    Journal: Cell Reports

    Article Title: The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37

    doi: 10.1016/j.celrep.2026.117146

    Figure Lengend Snippet: DAB1 overexpression induces axonal defects during development (A) Schematic representation of cerebellar and cerebellar-like structures in zebrafish. Granule cell (GC) axons, named parallel fibers (PFs), innervate Purkinje cells (PCs) in the molecular cell layer. Axons from GCs in eminentia granularis (EG) innervate a type of Purkinje-like neuron called crest cells (CrCs). (B) Representative confocal images of zebrafish larvae, from a GFP + cerebellar granule cell line, microinjected with control or human DAB1 mRNA at 5 dpf; axonal tracts of GCs from EG are indicated with orange (absent) or gray (present) arrows. (C) Percentage of present or absent GC axonal tracts that innervate PC-like neurons (graphic representation of data by mean ± SD of at least 20 embryos/condition/replicate; three independent experiments; ꭕ 2 test for the percentage of GC axonal tracts, present or absent; ∗∗∗ p < 0.001). (D) Schematics of muscle innervation by PMN axon tracts in zebrafish larvae at 24 hpf. (E) Representative confocal images (40× objective) of PMN axon tracts from the 6-somites-spanning region anterior to the cloaca from control or DAB1 mRNA-injected embryos; whole-mounted immunofluorescence with SV2 antibody, representative z-projection images (maximum intensity). (F) Length of zebrafish PMN axon tracts in control and DAB1 mRNA-microinjected embryos ( n = 23 embryos for control and n = 24 embryos in DAB1 mRNA condition; four independent experiments; independent t test, ∗ p < 0.05; data are represented by the mean ± SD). (G) Proposed mechanistic model showing disruption of DRL function by the (ATTTC) n , which hyperactivates the neurodevelopmental enhancer, leading to DAB1 upregulation and DAB1 protein accumulation in axons, likely contributing to SCA37. See also .

    Article Snippet: Embryos were incubated with anti-synaptic vesicle 2 (SV2) antibody (1:200, #AB2315387, Developmental Studies Hybridoma Bank) diluted in blocking solution overnight, at 4°C.

    Techniques: Over Expression, Control, Injection, Immunofluorescence, Disruption

    DAB1 overexpression induces axonal defects during development (A) Schematic representation of cerebellar and cerebellar-like structures in zebrafish. Granule cell (GC) axons, named parallel fibers (PFs), innervate Purkinje cells (PCs) in the molecular cell layer. Axons from GCs in eminentia granularis (EG) innervate a type of Purkinje-like neuron called crest cells (CrCs). (B) Representative confocal images of zebrafish larvae, from a GFP + cerebellar granule cell line, microinjected with control or human DAB1 mRNA at 5 dpf; axonal tracts of GCs from EG are indicated with orange (absent) or gray (present) arrows. (C) Percentage of present or absent GC axonal tracts that innervate PC-like neurons (graphic representation of data by mean ± SD of at least 20 embryos/condition/replicate; three independent experiments; ꭕ 2 test for the percentage of GC axonal tracts, present or absent; ∗∗∗ p < 0.001). (D) Schematics of muscle innervation by PMN axon tracts in zebrafish larvae at 24 hpf. (E) Representative confocal images (40× objective) of PMN axon tracts from the 6-somites-spanning region anterior to the cloaca from control or DAB1 mRNA-injected embryos; whole-mounted immunofluorescence with SV2 antibody, representative z-projection images (maximum intensity). (F) Length of zebrafish PMN axon tracts in control and DAB1 mRNA-microinjected embryos ( n = 23 embryos for control and n = 24 embryos in DAB1 mRNA condition; four independent experiments; independent t test, ∗ p < 0.05; data are represented by the mean ± SD). (G) Proposed mechanistic model showing disruption of DRL function by the (ATTTC) n , which hyperactivates the neurodevelopmental enhancer, leading to DAB1 upregulation and DAB1 protein accumulation in axons, likely contributing to SCA37. See also .

    Journal: Cell Reports

    Article Title: The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37

    doi: 10.1016/j.celrep.2026.117146

    Figure Lengend Snippet: DAB1 overexpression induces axonal defects during development (A) Schematic representation of cerebellar and cerebellar-like structures in zebrafish. Granule cell (GC) axons, named parallel fibers (PFs), innervate Purkinje cells (PCs) in the molecular cell layer. Axons from GCs in eminentia granularis (EG) innervate a type of Purkinje-like neuron called crest cells (CrCs). (B) Representative confocal images of zebrafish larvae, from a GFP + cerebellar granule cell line, microinjected with control or human DAB1 mRNA at 5 dpf; axonal tracts of GCs from EG are indicated with orange (absent) or gray (present) arrows. (C) Percentage of present or absent GC axonal tracts that innervate PC-like neurons (graphic representation of data by mean ± SD of at least 20 embryos/condition/replicate; three independent experiments; ꭕ 2 test for the percentage of GC axonal tracts, present or absent; ∗∗∗ p < 0.001). (D) Schematics of muscle innervation by PMN axon tracts in zebrafish larvae at 24 hpf. (E) Representative confocal images (40× objective) of PMN axon tracts from the 6-somites-spanning region anterior to the cloaca from control or DAB1 mRNA-injected embryos; whole-mounted immunofluorescence with SV2 antibody, representative z-projection images (maximum intensity). (F) Length of zebrafish PMN axon tracts in control and DAB1 mRNA-microinjected embryos ( n = 23 embryos for control and n = 24 embryos in DAB1 mRNA condition; four independent experiments; independent t test, ∗ p < 0.05; data are represented by the mean ± SD). (G) Proposed mechanistic model showing disruption of DRL function by the (ATTTC) n , which hyperactivates the neurodevelopmental enhancer, leading to DAB1 upregulation and DAB1 protein accumulation in axons, likely contributing to SCA37. See also .

    Article Snippet: Anti-Synaptic Vesicle 2 (SV2) primary antibody , Developmental Studies Hybridoma Bank , Cat #SV2;RRID:AB_2315387.

    Techniques: Over Expression, Control, Injection, Immunofluorescence, Disruption

    Motor function (latency to fall from an inverted screen) measured in 3-month-old WT, TDP43 Q331K/+ , LDHB MNKO and TDP43 Q331K/+ ; LDHB MNKO mice (A), electrophysiological recording performed on 3-month-old animals to determine the difference in CMAP at the ankle and sciatic notch ( B & C ) and Sensory Nerve Action Potential (SNAP) at the tail (n = 10-12) (D). NMJs on lumbrical muscles stained with anti-NF, anti-SV2 and BTX to compare innervation of 3-month-old mice of different genotypes (n = 3; statistical comparison is between fully innervated endplates). Statistical significance was determined by pairwise Fisher’s exact tests on raw counts, with a Bonferroni correction applied for multiple comparisons. (E-I). Spinal cords stained to detect TDP43 and nuclei (DAPI) indicate no mislocalization in 6-month-old LDHB MNKO; TDP43 Q331K/+ or LDHB MNKO mice (J & K) . Representative images of 40x and 63x toluidine blue-stained sections of tibial nerves from 3-month-old animals (L-O) . Average g-ratio indicates no difference in degeneration between genotypes (n = 3). Unless otherwise mentioned, all statistical significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (P) . ***p < 0.001, **p < 0.01.

    Journal: PLOS One

    Article Title: Dysregulated lactate metabolism synergizes with ALS genetic risk factors to accelerate motor decline

    doi: 10.1371/journal.pone.0347135

    Figure Lengend Snippet: Motor function (latency to fall from an inverted screen) measured in 3-month-old WT, TDP43 Q331K/+ , LDHB MNKO and TDP43 Q331K/+ ; LDHB MNKO mice (A), electrophysiological recording performed on 3-month-old animals to determine the difference in CMAP at the ankle and sciatic notch ( B & C ) and Sensory Nerve Action Potential (SNAP) at the tail (n = 10-12) (D). NMJs on lumbrical muscles stained with anti-NF, anti-SV2 and BTX to compare innervation of 3-month-old mice of different genotypes (n = 3; statistical comparison is between fully innervated endplates). Statistical significance was determined by pairwise Fisher’s exact tests on raw counts, with a Bonferroni correction applied for multiple comparisons. (E-I). Spinal cords stained to detect TDP43 and nuclei (DAPI) indicate no mislocalization in 6-month-old LDHB MNKO; TDP43 Q331K/+ or LDHB MNKO mice (J & K) . Representative images of 40x and 63x toluidine blue-stained sections of tibial nerves from 3-month-old animals (L-O) . Average g-ratio indicates no difference in degeneration between genotypes (n = 3). Unless otherwise mentioned, all statistical significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (P) . ***p < 0.001, **p < 0.01.

    Article Snippet: Following three rinses with Phosphate buffered saline (PBS), lumbrical muscles were dissected for staining with anti-SV2 (Developmental Studies Hybridoma Bank AB2315387, 1:200), anti-2H3 (Developmental Studies Hybridoma Bank AB2314897, 1:100) and α-bungarotoxin (BTX) (Biotium 00006, 1:500) as described previously [ ].

    Techniques: Muscles, Staining, Comparison